rhil 4  (R&D Systems)

Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: The cuproptosis blocker SS-31 ameliorated airway responsiveness and airway inflammation in chronic asthma model mice (n = 10 mice per group). ( A ) Ers, Rrs and Rn in different groups challenged with aerosol Mch (mg/ml). ( B ) Representative images of HE-stained lung tissue. C. Cell classification (eosinophils, lymphocytes, and neutrophils) and counts in BALF. D. The levels of the inflammatory factors IL-4, TGFβ1 and IL-1β in the BALF. The data are shown as the means ± SEMs, n = 10. One-way analysis of variance (ANOVA) and the Student–Newman–Keuls (SNK) method were used, and P < 0.05 was considered to indicate statistical significance. *p < 0.05 for the OVA group vs. the control group. # p < 0.05 for the SS-31 + OVA group vs. the PBS + OVA group. Above experiment was repeated three times.

Article Snippet: When the cells reached 60–80% confluence, the BEAS-2B cells were incubated with recombinant human IL-4 (rhIL-4) and TGF-β1 (10 ng/mL, R&D Systems) for 24 h to stimulate airway remodeling in vitro.

Techniques: Aerosol, Staining, Control

Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: Cuproptosis was increased in BEAS-2B cells incubated with rhIL-4 and TGFβ1 and the addition of SS-31 reversed the above process. ( A ) Quantification of the effects of different concentrations of SS-31 on the cell viability of BEAS-2B cells. ( B ) Cu 2+ levels in the supernatant of BEAS-2B cells. ( C ) Cu 2+ levels in the cytoplasm of BEAS-2B cells. D. Protein expression of cuproptosis-related genes (FDX-1, LIAS, and DLAT) in BEAS-2B cells. ( D ) Quantification of the protein expression levels of cuproptosis-related genes in BEAS-2B cells. The data are shown as the means ± SEMs. *p < 0.05 for the rhIL-4 + TGFβ1 PBS group vs. the rhIL-4 + TGFβ1 group. # p < 0.05 for the SS-31 + rhIL-4 + TGFβ1 group vs. the PBS + rhIL-4 + TGFβ1 group. Above experiment was repeated fourth times.

Article Snippet: When the cells reached 60–80% confluence, the BEAS-2B cells were incubated with recombinant human IL-4 (rhIL-4) and TGF-β1 (10 ng/mL, R&D Systems) for 24 h to stimulate airway remodeling in vitro.

Techniques: Incubation, Expressing

Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: Silencing FDX-1 attenuated cell remodeling in BEAS-2B cells incubated with rhIL-4 and TGFβ1. ( A , B ) Quantification of FDX-1 protein expression in BEAS-2B cells transfected with different siRNAs. ( C ) Cell morphology in different groups was observed under a light microscope. ( D , E ) Quantification of the effect of FDX-1 knockdown on cell migratory function. F. Effects of FDX-1 knockdown on the protein expression of EMT-related MMP-2, MMP-9, E-cadherin, N-cadherin, vimentin and smooth muscle-related α-SMA in BEAS-2B cells. The data are shown as the means ± SEMs. *p < 0.05 for the rhIL-4 + TGFβ1 PBS group vs. the rhIL-4 + TGFβ1 group. # p < 0.05 for the Scr + rhIL-4 + TGFβ1 group vs. the Si + rhIL-4 + TGFβ1 group. Above experiment was repeated fourth times.

Article Snippet: When the cells reached 60–80% confluence, the BEAS-2B cells were incubated with recombinant human IL-4 (rhIL-4) and TGF-β1 (10 ng/mL, R&D Systems) for 24 h to stimulate airway remodeling in vitro.

Techniques: Incubation, Expressing, Transfection, Light Microscopy, Knockdown